成人色图库,粗喘娇吟1V1军婚H,欧美午夜精品久久久,国产欧美日韩A片免费软件,成人欧美一区二区三区黑人免费,欧美日韩一区二区三区在线,国产真人做爰视频免费,久久精品天天爽夜夜爽,国产对白精品刺激一区二区,日本久久久久久级做爰片

發表期刊：Materials Today Bio

發表時間：2025

Abstract:

The development of efficient methods for sensing αlpha-glucosidase (α-Glu) and screening its inhibitors has attracted significant attention due to their pivotal role in discovering therapeutic medicines for Type 2 diabetes. Herein, a low-cost and sensitive fluorometric strategy based on red carbon dots (R-CDs) and cobalt oxyhydroxide nanosheets (CoOOH NSs) had been established to detect α-Glu and screen its inhibitory compounds in natural products. As a switched fluorescence source, the fluorescence of R-CDs at 625 nm could be quenched by CoOOH NSs via F?rster resonance energy transfer (FRET), assembled into nonfluorescent R-CDs@CoOOH nanocompositecomposites (R-CDs@CoOOH NCs). α-Glu hydrolyzed L-ascorbic acid-2-O-α-D-glucopyranose to produce ascorbic acid, which could reduce CoOOH NSs to Co2+, destroying R-CDs@CoOOH NCs and restoring the emission of red fluorescence. The proposed method exhibited a linear α-Glu range from 0.01 to 15 U mL?1 and a low limit of detection (LOD) of 0.0037 U mL?1. Meanwhile, high-performance liquid chromatography-DAD-fraction collector (HPLC-DAD-FC) had been employed and combined with ultra-high-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry to isolate, enrich, and characterize compounds from Polygonum cuspidatum (PC). This strategy was further extended by integrating the fluorometric platform with the HPLC-DAD-FC system to explore the inhibitory effects of PC extracts and anti-diabetic ingredients. Finally, 85 constituents were identified, with seven compounds exhibiting high α-Glu inhibitory activity. Consequently, the established strategy could accurately determine α-Glu in vitro and screen its inhibitors from natural products.

https://doi.org/10.1016/j.mtbio.2025.102018